Cell Line

BCRC Number: 60430 Add To Bookmark
Name: THP-1
Type: human acute monocytic leukemia
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 1 year
Gender: Male
Tissue: leukemia; acute monocytic; monocyte

Morphology: round
Split Ratio: maintain cell density between 105 to 106 cells/ml; do not allow the cell concentration to exceed 1 x 106cells/ml
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: negative
Receptor expressed: Fc; complement (C3b)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate,90%; Fetal bovine serum,10% supplemented with 0.05 mM 2-mercaptoethanol
Growth Property: suspension
SubCulture Procedure: addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1-2 x 105 cells/ml
Medium Renewal: every 2 to 3 days

Description: derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia; the cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins; the cells stain positive for alpha-naphthyl butyrate esterase, produce lysozymes and are phagocytic (both latex beads and sensitized erythrocytes; the cells restore the response of purified T lymphocytes to Con A, show increased CO2 production on phagocytosis and can be differentiated into macrophage-like cells
Others: STR-PCR profile — D7S820:10 CSF1PO:11, 13 TH01:8, 9.3 D13S317:13 D16S539:11, 12 vWA:16 TPOX:8, 11 Amelogenin:X, Y D5S818:11, 12
Product Character: lysozyme

Image / Description:   200倍視野下高細胞密度

Biosafety Level: 1
Reference: Int. J. Cancer 26: 171-176, 1980; J. Immunol. 131: 1882-1888, 1983
growing cell in 15ml tube for all NTD$ 6000 Order this item

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