Cell Line



BCRC Number: 60429
Name: MDA-MB-453
Type: human breast carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 48 years
Gender: Female
Tissue: breast; mammary gland

Morphology: epithelial
Split Ratio: 1:2 to 1:6
Mycoplasma Test: negative
Cytogenetics: hypo- to near-tetraploid range; modal number = 90
Tumorigenic: no; The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Receptor expressed: fibroblast growth factor (FGF)

Incubation Temp(°C): 37°C
Incubation Condition: without CO2 in air
Culture Medium: Leibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from an effusion of a 48-year-old female patient with metastatic carcinoma of the breast, involving the nodes , brain and both pleural and pericardial cavities ; the cells overexpress FGF receptors
Others: STR-PCR profile — D7S820:10 CSF1PO:10, 12 TH01:6 D13S317:12 D16S539:9 vWA:17, 18 TPOX:10 Amelogenin:X D5S818:11

Image / Description:   100倍視野下
  200倍視野下
 

Biosafety Level: 1
Reference: In Vitro 14: 911-915, 1978; Cancer Res. 39: 919-922, 1979
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