Cell Line



BCRC Number: 60425
Name: MDA-MB-231
Type: human breast adenocarcinoma(grade III)
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 51 years
Gender: Female
Tissue: breast; mammary gland; adenocarcinoma; pleural effusion

Morphology: epithelial
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: Negative
Cytogenetics: aneuploid
Tumorigenic: yes, in nude mice also in ALS treated BALB/c mice; forms poorly differentiated adenocarcinoma (grade III)
Receptor expressed: epidermal growth factor (EGF); transforming growth factor alpha (TGF alpha)

Incubation Temp(°C): 37°C
Incubation Condition: without CO2 in air
Culture Medium: Leibovitz's L-15 medium with 2 mM L-glutamine,90%;fetal bovine serum,10%.
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:8, 9 CSF1PO:12, 13 TH01:7, 9.3 D13S317:13 D16S539:12 vWA:15, 18 TPOX:8, 9 Amelogenin:X D5S818:12

Image / Description:   100倍視野下低細胞密度
  100倍視野下高細胞密度
 

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 53: 661-674, 1974
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