Cell Line



BCRC Number: 60424
Name: Jurkat, Clone E6-1
Type: Human acute T cell leukemia
Species: Homo sapiens (human)
Gender: Male
Tissue: leukemia; acute T cell; T lymphocyte

Morphology: lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml; do not allow the cell concentration to exceed 3 x 106 cells/ml
Mycoplasma Test: Negative
Cytogenetics: pseudodiploid
Doubling time: 48hrs
Receptor expressed: T cell antigen receptor

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate,90%;fetal bovine serum,10%.
Growth Property: suspension
SubCulture Procedure: Addition or replacement of fresh medium.
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1 x 10 5 cells/ml
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Description: This is a clone of the Jurkat-FHCRC cell line, a derivative of the Jurkat cell line which was established from the peripheral blood of a 14 year old boy ; the clone E6-1 cells produce large amounts of IL-2 after stimulation with phorbol esters and either lectins or monoclonal antibodies against the T3 antigen (both types of stimulants are needed to induce IL-2 production)
Others: STR-PCR profile — D7S820:8, 12 CSF1PO:11, 12 TH01:6, 9.3 D13S317:8, 12 D16S539:11 vWA:18 TPOX:8, 10 Amelogenin:X, Y D5S818:9
Product Character: interleukin-2 (interleukin 2, IL-2) gamma interferon

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Biosafety Level: 1
Reference: J. Immunol. 133: 123-128, 1984
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