Cell Line

BCRC Number: 60422 Add To Bookmark
Name: Y79
Type: human retinoblastoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 2.5 years
Gender: Female
Tissue: eye; retina; retinoblastoma

Morphology: multicellular clusters
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: negative
Cytogenetics: hypertriploidy, with abnormalities including dicentrics, breaks, pulverizations and minutes

Incubation Temp(°C): 37°C
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate,80%;fetal bovine serum,20%.
Growth Property: suspension
SubCulture Procedure: Allow aggregates to settle to the bottom of the flask. Remove supernatant and discard. Add fresh medium, aspirate and dispense into new flasks.
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: isolated by explant culture of a primary tumor from the right eye obtained immediately after enucleation; the donor had a strong maternal family history of retinoblastoma; ultrastructural features including nuclear membrane infoldings, triple membrane structures, microtubules, large coated vesicles, centrioles, basal bodies and annulate lamellae were reportedly similar to those of the original tumor
Others: STR-PCR profile — D7S820:8, 9 CSF1PO:11, 12 TH01:6, 9.3 D13S317:11, 12 D16S539:13, 14 vWA:15, 18 TPOX:8 Amelogenin:X D5S818:11, 12

Image / Description:   100倍視野下
  100倍視野下 高細胞密度
  200倍視野下 高細胞密度

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 53: 347-360, 1974
Frozen Vial for all NTD$ 6000 Order this item

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