Cell Line

BCRC Number: 60420 Add To Bookmark
Name: SJCRH30 RC13; RMS 13; SJRH30
Type: human rhabdomyosarcoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 17 years
Gender: unknown
Tissue: muscle; rhabdomyosarcoma; metastatic site: bone marrow

Morphology: fibroblast
Split Ratio: 1:5 to 1:10
Mycoplasma Test: negative
Cytogenetics: double minutes are present; a translocation specific for rhabdomyosarcoma t(2;13)(q35;q14) is present

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: established from cells from the bone marrow of a child with rhabdomyosarcoma; the cells show ultrastructural elements of primitive skeletal muscle differentiation; oncogene expressed: gli + (amplified 30 fold), N-myc + (not amplified)
Others: STR-PCR profile — D7S820:10 CSF1PO:10, 11 TH01:9, 9.3 D13S317:11 D16S539:12 vWA:17, 18 TPOX:8, 11 Amelogenin:X, Y D5S818:12, 13

Image / Description:   100倍視野下

Biosafety Level: 1
Reference: Nature 358: 80-83, 1992; Cancer Res. 49: 5407-5413, 1989; Cytogenet. Cell Genet. 45: 148-155, 1987
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