Cell Line



BCRC Number: 60419 Add To Bookmark
Name: MH-S
Type: mouse alveolar macrophage
Species: Mus musculus (mouse)
Strain Ethnicity: BALB/c
Age Stage: 7 weeks
Gender: Male
Tissue: macrophage, alveolar; lung

Morphology: macrophage
Split Ratio: 1:2 to 1:6
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: negative
Receptor expressed: Fc

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol,90%;fetal bovine serum,10%.
Growth Property: mixed: adherent and suspension
SubCulture Procedure: floating cells: centrifugation; attached cells: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days; cultures become acidic quickly

Description: The MH-S cell line was derived by SV40 transformation of an adherent cell enriched population of mouse alveolar macrophages; the cells retain many of the properties of alveolar macrophages including typical macrophage morphology; they are adherent, phagocytic, esterase-positive and peroxidase-negative; the cells express intracellular T-antigen and cell surface Ia and Mac-1 antigens; the cells are Fc receptor-positive; they produce IL-1 constitutively; lipopolysaccharide (LPS) treatment stimulates IL-1 production; the cells can suppress the in vitro plaque forming cell (PFC) response in a cell dose dependent manner
Product Character: interleukin 1 (IL-1)

Image / Description:   200倍視野下
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Biosafety Level: 1
Reference: J. Leukocyte Biol. 46: 119-127, 1989
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