Cell Line



BCRC Number: 60415 Add To Bookmark
Name: RMC
Type: rat kidney mesangial cell, immortalized with pSV3-Neo
Species: Rattus norvegicus (rat)
Strain Ethnicity: Sprague-Dawley
Age Stage: 3 months
Gender: Male
Tissue: kidney; mesangial; immortalized with pSV3-Neo

Morphology: epithelial
Split Ratio: 1:4 to 1:8
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 85% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.4 mg/ml G418 + 15% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Rat mesangial cell at passage 8 were immortalized with pSV3-Neo and maintained in the presence of G418; the cells express normal genes of the wild type mesangial cells; they are positive for desmin and vimentin and negative for cytokeratin 8; mesangial cells are crucial for kidney function and are used extensively in kidney research
Product Character: desmin; vimentin

Image / Description:   200倍視野下
 

Biosafety Level: 1
Shipping/Field/Price:
Frozen Vial for all NTD$ 7000 Order this item

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