Cell Line

BCRC Number: 60410
Name: RIN-m5F
Type: rat insulinoma
Species: Rattus norvegicus (rat)
Strain Ethnicity: NEDH
Gender: Male
Tissue: pancreas; islet of Langerhans; beta cell; insulinoma

Morphology: epithelial
Split Ratio: 1:3 to 1:6
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate,90%;fetal bovine serum,10%.
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: derived from the RIN-m rat islet cell line; the cells produce and secrete insulin, and produce L-dopa-decarboxylase; unlike the parental line they do not produce somatostatin
Product Character: insulin; L-dopa-decarboxylase

Image / Description:   200倍視野下
  100倍視野下 高細胞密度
  200倍視野下 高細胞密度

Biosafety Level: 1
Patent Statement: This material is cited in a U.S. and/or other Patent application and may not be used to infringe the patent claims.
Reference: Proc. Soc. Exp. Biol. Med. 175: 35-38, 1984; Proc. Natl. Acad. Sci. USA 74: 628-632, 1977; Diabetes 31: 521-531, 1982; Proc. Natl. Acad. Sci. USA 77: 3519-3523, 1980; Endocrinology 112: 1070-1075, 1983
Frozen Vial for all NTD$ 6000 Order this item

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