Cell Line

BCRC Number: 60406 Add To Bookmark
Name: 1A3
Type: mouse hybridoma, anti HeLa cell
Species: Mus musculus (mouse)
Strain Ethnicity: BALB/c (B cell); BALB/c (myeloma)
Gender: unknown
Tissue: hybridoma; B lymphocyte

Morphology: lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Dulbecco's modified Eagle's medium with 4mM L-glutamine, 4.5 g/L glucose,80%;fetal bovine serum,20%.
Growth Property: suspension
SubCulture Procedure: addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1-2 x 105 cells ml
Medium Renewal: every 2 to 3 days

Description: Formed by the fusion of P3X63Ag8 mouse myeloma cells with spleen cells from BALB/c mice immunized with HeLa cells; the cells produce monoclonal antibody specific for HeLa cells.
Product Character: immunoglobulin; monoclonal antibody; against HeLa cells

Image / Description:   200倍視野下

Biosafety Level: 1
Patent Statement: U.S. Pat. 4,618,585
Reference: U.S. Pat. 4,618,585 dated Oct. 21, 1986
Frozen Vial for all NTD$ 6000 Order this item

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