Cell Line

BCRC Number: 60405 Add To Bookmark
Name: L-M
Type: mouse connective tissue, derivative of NCTC clone 929
Species: Mus musculus (mouse)
Strain Ethnicity: C3H/An
Age Stage: 100 days
Gender: Male
Tissue: subcutaneous connective tissue; normal; areolar and adipose

Morphology: fibroblast
Split Ratio: 1:2 to 1:8
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: negative
Virus resistance: poliovirus 1, 2, 3

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Medium 199 with Earle's BSS supplemented with 0.5% Bacto-peptone
Growth Property: adherent
SubCulture Procedure: by scraping
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: derived from NCTC clone 929 (see BCRC 60091); the line is characterized by its propagation in a serum-free medium and by marker chromosomes

Image / Description:   200倍視野下

Biosafety Level: 1
Reference: Proc. Soc. Exp. Biol. Med. 92: 803-806, 1956; Exp. Cell Res. 17: 490-498, 1959; Proc. Soc. Exp. Biol. Med. 110: 194-198, 1962; J. Natl. Cancer Inst. 26: 1075-1083, 1961
Frozen Vial for all NTD$ 6000 Order this item

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