Cell Line

BCRC Number: 60385 Add To Bookmark
Name: ARH-77
Type: human plasma cell leukemia, IgG secreting
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 33 years
Gender: Female
Tissue: leukemia; plasma cell; B lymphoblast; Epstein-Barr virus (EBV) transformed

Morphology: lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: negative
Cytogenetics: hypodiploid

Incubation Temp(°C): 37°C
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 10 mM HEPES, 1 mM sodium pyruvate, 4.5 g/L glucose, 1.5 g/L sodium bicarbonate,90%;fetal bovine serum,10%.
Growth Property: suspension
SubCulture Procedure: addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1-4 x 105 cells/ml
Medium Renewal: every 2 to 3 days depending on cell density

Description: established from the peripheral blood of a patient with IgG plasma cell leukemia; the cells synthesize IgG1 (kappa-light-chains); although established from cells taken from a patient with a plasma cell leukemia, this line has been shown to be an EBV-transformed B lymphoblastoid cell line; the cells are positive for Epstein-Barr nuclear antigen(EBNA +) and Epstein-Barr viral capsid antigen (EBVCA +); the line has been cured of a previous mycoplasma infection
Others: STR-PCR profile — D7S820:7, 12 CSF1PO:6, 10 TH01:8, 9.3 D13S317:11, 13 D16S539:9, 13 vWA:17 TPOX:8 Amelogenin:X D5S818:10, 13
Product Character: immunoglobulin; isotype: IgG1, kappa light chain

Image / Description:   200倍視野下高細胞密度

Biosafety Level: 2
Reference: Proc. Am. Assoc. Cancer Res. 19: 211, 1978; Cancer Res. 38: 2508-2513, 1978
Frozen Vial for all NTD$ 6000 Order this item

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