Cell Line



BCRC Number: 60384
Name: RPMI 8226
Type: human myeloma
Species: Homo sapiens (human)
Age Stage: 61 years
Gender: Male
Tissue: myeloma; plasmacytoma; B lymphocyte

Morphology: lymphoblast
Split Ratio: Maintain cell density between 5 x 105 and 2 x 106 viable cells/mL.
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: negative
Cytogenetics: triploid;modal number=68 to 70
Virus susceptibility: poliovirus 1; vesicular stomatitis (Indiana); herpes simplex; vaccinia

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 10 mM HEPES, 1 mM sodium pyruvate, 4.5 g/L glucose, 1.5 g/L sodium bicarbonate,80%;fetal bovine serum,20%.
Growth Property: suspension
SubCulture Procedure: by the addition of fresh medium or replacement of medium. Maintain cell density between 5 x 105 and 2 x 106 viable cells/mL. Do not allow the cell density to exceed 2 to 3 x 106 cells/mL.
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 5 x 105 cells/ml
Medium Renewal: every 2 to 3 days

Description: derived from the peripheral blood of a 61-year-old male with multiple myeloma; the cells produce and secrete free lambda-type light chains of immunoglobulin; there is no evidence of heavy chain production (cytoplasmic or secreted)
Others: STR-PCR profile — D7S820:9, 10 CSF1PO:12 TH01:8 D13S317:11 D16S539:9 vWA:16, 18 TPOX:8, 11 Amelogenin:X, Y D5S818:11, 13
Product Character: immunoglobulin light chain; isotype: lambda light chain

Image / Description:   200倍視野下高細胞密度
 

Biosafety Level: 1
Reference: Proc. Soc. Exp. Biol. Med. 125: 1246-1250, 1967; N.Y. State J. Med. 68: 2054-2060, 1968
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