Cell Line



BCRC Number: 60383
Name: ARPE-19
Type: human retinal pigmented epithelium
Species: Homo sapiens (human)
Age Stage: 19 years
Gender: Male
Tissue: eye; retina; retinal pigmented epithelium; normal

Morphology: epithelial
Split Ratio: 1:3 to 1:5
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: negative
Cytogenetics: diploid

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate,90%;fetal bovine serum,10%.
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: this is a spontaneously arising retinal pigment epithelia (RPE) cell line derived from the normal eyes of 19-year-old male who died from head trauma in a motor vehicle accident; the cells form stable monolayers, which exhibit morphological and functional polarity; the line expresses the RPE-specific markers CRALBP and RPE-65; the cells exhibit morphological polarization when plated on laminin-coated Transwell-COL filters in medium with a low serum concentration; they form tight-junctions with transepithelial resistance of monolayers reaching a maximum of 50 to 100 ohms/cm2 after 4 weeks of culture; the cells are diploid and can be carried for over 30 passages.
Others: STR-PCR profile — D7S820:9, 11 CSF1PO:11 TH01:6, 9.3 D13S317:11, 12 D16S539:9, 11 vWA:16, 19 TPOX:9, 11 Amelogenin:X, Y D5S818:13

Image / Description:   100倍視野下高密度細胞
  200倍視野下高密度細胞
 

Biosafety Level: 1
Reference: Exp. Eye Res. 62: 155-169, 1996
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