Cell Line



BCRC Number: 60382
Name: M059J
Type: human glioblastoma
Species: Homo sapiens (human)
Age Stage: 33 years
Gender: Male
Tissue: brain; glioblastoma; malignant; glial cell

Morphology: fibroblast
Split Ratio: 1:6 to 1:8
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: negative
Cytogenetics: aneuploid

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: A 1:1 mixture of Dulbecco's Modified Eagle's Medium and Ham's F12 medium with 2.5 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0. 5 mM sodium pyruvate, 0.05 mM non-essential amino acids,90%;fetal bovine serum,10%.
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: isolated from a tumor specimen taken from a patient with untreated malignant glioblastoma; the cells were isolated concurrently from the same tumor specimen as M059K (see BCRC 60381); M059J cells lack DNA-dependent protein kinase activity, while M059K cells express normal levels of DNA-dependent protein kinase M059J cells are approximately 30-fold more sensitive to ionizing radiation than M059K cells; M059J cells are more sensitive than M059K cells to the cytotoxic effects of bleomycin, N,N-bis(2-choroethyl)-N-nitrosourea and nitrogen mustard M059J cells are deficient in repair of DNA double strand breaks; the cells are negative for glial fibrillary acidic protein (GFAP); M059K and M059J provide a useful model system in which to study the role of DNA protein kinase in cellular and molecular processes involving DNA damage recognition and repair.
Others: STR-PCR profile — D7S820:10 CSF1PO:10, 12 TH01:9.3 D13S317:14 D16S539:10, 12 vWA:17 TPOX:8 Amelogenin:X, Y D5S818:11, 12

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Biosafety Level: 1
Reference: Radiat. Res. 134: 349-354, 1993; Science 267: 1183-1185, 1995
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