Cell Line



BCRC Number: 60381
Name: M059K
Type: human glioblastoma
Species: Homo sapiens (human)
Age Stage: 33 years
Gender: Male
Tissue: brain; glioblastoma; malignant; glial cell

Morphology: fibroblast
Split Ratio: 1:6 to 1:8
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: negative
Cytogenetics: modal Chromosome Number = 75 with a range of 65 to 79
Tumorigenic: yes, forms tumors in SCID mice

Incubation Temp(°C): 37°C
Culture Medium: A 1:1 mixture of Dulbecco's Modified Eagle's Medium and Ham's F12 medium with 2.5 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0. 5 mM sodium pyruvate, 0.05 mM non-essential amino acids,90%;fetal bovine serum,10%.
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:10 CSF1PO:10, 12 TH01:9.3 D13S317:14 D16S539:10, 12 vWA:17 TPOX:8 Amelogenin:X, Y D5S818:11, 12

Image / Description:   200倍視野下
  100倍視野下高細胞密度
  200倍視野下高細胞密度
  100倍視野下
  200倍視野下
 

Biosafety Level: 1
Reference: Radiat. Res. 134: 349-354, 1993; Science 267: 1183-1185, 1995
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