Cell Line

BCRC Number: 60380 Add To Bookmark
Name: DBTRG-05MG
Type: human glioblastoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 59 years
Gender: Female
Tissue: brain; glioblastoma; glial cell

Morphology: fibroblast
Split Ratio: 1:3 to 1:4
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: negative
Cytogenetics: near tetraploid; range 87 to 91; most cells were missing copies of chromosome 10 and had extra copies of chromosome 7
Receptor expressed: epidermal growth factor (EGF)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 10 mg/L adenine, 1 mg/L adenosine triphosphate, 100 mg/L L-cytine, 5950 mg/L HEPES, 15 mg/L hypoxanthine, 50 mg/L L-isoleucine, 50 mg/L L-proline, 100 mg/L sodium pyruvate and 1 mg/L thymidine,90%;fetal bovine serum,10%.
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: The DBTRG-O5MG (Denver Brain Tumor Research Group 05) cell line was established from tissue from a patient with glioblastoma multiforme who had been treated with local brain irradiation and multidrug chemotherapy; the cells are negative for platelet derived growth factor (PDGF), neuronal cell adhesion molecule (NCAM), glial fibrillary acid protein (GFAP) and class II antigen (HLA DR); no loss of heterozygosity in the p53 tumor suppressor gene was detected.
Others: STR-PCR profile — D7S820:11 CSF1PO:10, 11 TH01:7, 8 D13S317:9 D16S539:10, 12 vWA:15, 16 TPOX:8 Amelogenin:X D5S818:11, 13
Product Character: vimentin; S-100 protein; neuron specific enolase

Image / Description:   100倍視野下高細胞密度

Biosafety Level: 1
Reference: In Vitro Cell. Dev. Biol. 28A: 609-614, 1992; Cancer Res. 57: 5391-5398, 1997; J. Biol. Chem. 271: 22428-22433, 1996
Frozen Vial for all NTD$ 6000 Order this item

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