Cell Line



BCRC Number: 60376 Add To Bookmark
Name: CTLL-2
Type: mouse cytotoxic T cells
Species: Mus musculus (mouse)
Strain Ethnicity: C57BL/6
Gender: unknown
Tissue: T lymphocytes; cytotoxic T lymphocyte

Morphology: lymphoblast
Split Ratio: 1:100 to 1:1000
Mycoplasma Test: negative
Receptor expressed: interleukin 2 (IL-2)

Incubation Temp(°C): 37°C
Culture Medium: RPMI1640 (with 2mM L-glutamine, 10mM HEPES, 1mM sodium pyruvate, 4.5 g/L glucose, 1.5 g/L sodium bicarbonate), supplemented 10 % fetal bovine serum, 10% rat T-STIM factor with Con A (available from Becton Dickinson, Cat#354115) , 2mM L-glutamine, 1mM sodium pyruvate.
Growth Property: suspension
SubCulture Procedure: addition or replacement of fresh medium; NOTE: Subculture actively growing suspension cultures before they the reached 1 X 105 cell/ml. Do not allow cells to exceed 1 X 105 cells/ml or IL-2 will be rapidly depleted and the cells will quickly lose viability.
Freezed Medium: 82.5% culture medium + 10% additional fetal bovine serum + 7.5% DMSO
Seeding Density: 100 to 1000 cells/ml
Medium Renewal: every 2 to 3 days

Description: This line is a clone of cytotoxic T cells derived from a C57BL/6 mouse; the cells are dependent upon IL-2 for growth, and can be used to assay for IL-2; immediately after thawing (or after overgrowth of a culture) the culture will appear to have few or no

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Nature 268: 154-156, 1977
Shipping/Field/Price:
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