Cell Line

BCRC Number: 60373 Add To Bookmark
Name: NCI-H460
Type: human lung large cell carcinoma
Species: Homo sapiens (human)
Gender: Male
Tissue: lung; large cell carcinoma; pleural effusion

Morphology: epithelial
Split Ratio: 1:3 to 1:8
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: Negative
Cytogenetics: hypotriploid; modal number:57-58
Tumorigenic: yes; forms tumors after subcutaneous injection into nude mice
Doubling time: 23 hrs in medium with serum; 42 to 60 hrs in serum-free medium

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 10 mM HEPES, 1 mM sodium pyruvate, 4.5 g/L glucose, 1.5 g/L sodium bicarbonate,90%;fetal bovine serum,10%.
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: derived from the pleural fluid of a patient with large cell carcinoma prior to therapy; the cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities; the cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein; the line expresses some properties of neuroendocrine cells, is relatively chemosensitive and can be cloned in soft agar (with or without serum)
Others: STR-PCR profile — D7S820:9, 12 CSF1PO:11, 12 TH01:9.3 D13S317:13 D16S539:9 vWA:17 TPOX:8 Amelogenin:X, Y D5S818:9, 10

Image / Description:   200倍視野下低細胞密度

Biosafety Level: 1
Reference: Cancer Res. 45: 1187-1197, 1985; Science 246: 491-494, 1989; Cancer Res. 46: 798-806, 1986
Frozen Vial for all NTD$ 6000 Order this item

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