Cell Line



BCRC Number: 60367 Add To Bookmark
Name: A-253
Type: Human submaxillary salivary gland, epidermoid carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 54 years
Gender: unknown
Tissue: submaxillary salivary gland; epidermoid carcinoma

Morphology: epithelial
Split Ratio: 1:2 to 1:4
Mycoplasma Test: negative
Cytogenetics: near triploid with at least 6 markers
Tumorigenic: yes; in nude mice; forms undifferentiated carcinoma; tumors also develop in anti thymocyte serum treated newborn mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% McCoy's 5a medium (modified) + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% Culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:10, 12 CSF1PO:12, 13 TH01:6, 8 D13S317:9, 12 D16S539:12 vWA:16 TPOX:8, 12 Amelogenin:X D5S818:11

Image / Description:   100倍視野下
 

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 51: 1417-1423, 1973; J. Natl. Cancer Inst. 59: 221-226, 1977
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