Cell Line



BCRC Number: 60366 Add To Bookmark
Name: SV40 MES 13
Type: mouse glomerular mesangial cells
Species: Mus musculus (mouse)
Strain Ethnicity: (C57BL/6J x SJL/J)F1
Age Stage: 7 to 10 weeks
Gender: unknown
Tissue: kidney; glomerulus; mesangial cell

Morphology: myofibroblast-like
Split Ratio: 1:4 to 1:10
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: A 3:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium,95%; fetal bovine serum,5%; supplemented with 14 mM HEPES .
Growth Property: adherent
SubCulture Procedure: Trypsin-EDTA
Freezed Medium: 93% Culture medium + 7% DMSO
Medium Renewal: Every 2 to 3 days

Description: established from the kidney of a mouse transgenic for the SV40 early region; the cells display prominent cytoskeletal staining for actin, and have abundant parallel fibrils in the cytoplasm; the cells will contract in the presence of 1 X 10(-6)M angiotensin II; the cells form colonies in soft agar, and are positive for SV40 large T antigen; they are negative for cytokeratins and Factor VIII related antigen

Image / Description:   200倍視野下低細胞密度
  100倍視野下低細胞密度
  100X視野下高細胞密度
  200X視野下高細胞密度
 

Biosafety Level: 1
Reference: Kidney Int. 33: 677-684, 1988
Shipping/Field/Price:
Frozen Vial for all NTD$ 7000 Order this item

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