Cell Line



BCRC Number: 60364
Name: HEP G2/2.2.1
Type: human hepatoblastoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 15 years
Gender: Male
Tissue: liver; hepatocellular carcinoma; transfected with a CYP7 minigene/luciferase construct

Morphology: epithelial
Split Ratio: 1:4 to 1:6
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: negative
Doubling time: These cells are slow to attach after subculture. Allow 4-5 days for reattachment.

Incubation Temp(°C): 37°C
Culture Medium: 90% of a 1:1 ration mixture of DMEM and Ham’s F12 medium+10%fetal bovine serum;supplemented with 0.4 mg/ml G418
Growth Property: adherent
SubCulture Procedure: Trypsin-EDTA
Freezed Medium: 93% Culture medium + 7% DMSO
Medium Renewal: Every 2 to 3 days

Description: derived from the hepatocellular carcinoma cell line HepG2 (see BCRC RM60025); the parental cells were stably tranfected at passage 48 with a human cholesterol 7 alpha-hydroxylase (CYP7) minigene/Luciferase construct
Others: STR-PCR profile — D7S820:10 CSF1PO:10, 11 TH01:9 D13S317:9, 13 D16S539:12, 13 vWA:17 TPOX:8, 9 Amelogenin:X, Y D5S818:11, 12

Image / Description:   200倍視野下
  100X視野下細胞高密度時生長形態
  200X視野下細胞高密度時生長形態
 

Biosafety Level: 1
Patent Statement: U.S. Pat. 5,821,057
Reference: U.S. Pat. 5,821,057 dated Oct. 13, 1998
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