Cell Line



BCRC Number: 60360
Name: U-87 MG
Type: human glioblastoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 44 years; grade III
Gender: Female
Tissue: brain; glioblastoma; astrocytoma

Split Ratio: 1:2 to 1:5
Mycoplasma Test: Negative
Cytogenetics: hypodiploid
Tumorigenic: yes, in nude mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: This is one of a number of cell lines derived from malignant gliomas by J. Ponten and associates from 1966 to 1969.
Others: STR-PCR profile — D7S820:8, 9 CSF1PO:10, 11 TH01:9.3 D13S317:8, 11 D16S539:12 vWA:15, 17 TPOX:8 Amelogenin:X D5S818:11, 12
Product Character: Notification : According to the Science Translation Medicine (2016, 8(354):1-4) report, the widely used glioma cell line U87MG is different from that of the original cells, which was first established at Uppsala University at 1966. That it is likely to be a bona fide human glioblastoma cell line of unknown origin.

Image / Description:   100倍視野下
  200倍視野下
 

Biosafety Level: 1
Reference: Sci Transl Med. 8: 1-4, 2016; Hum. Hered. 21: 238-241, 1971; Acta Pathol. Microbiol. Scand. 74: 465-486, 1968
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