Cell Line



BCRC Number: 60359
Name: BT-474
Type: human ductal carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 60 years
Gender: Female
Tissue: breast; mammary gland; ductal carcinoma

Morphology: epithelial
Split Ratio: 1:2 to 1:3
Mycoplasma Test: Negative
Cytogenetics: aneuploid
Virus susceptibility: mouse mammary tumor virus (RIII-MuMTV)
Tumorigenic: yes, in nude mice; forms nodules in Amsterdam/IMR rats with regression in 10 days

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Hybri-Care Medium (Modified Dulbecco's medium) with 30 ng/ml EGF+10% fetal bovine serum
Growth Property: Adherent patches of epithelial cells; compact, multilayered colonies, rarely become confluent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:9, 12 CSF1PO:10, 11 TH01:7 D13S317:11 D16S539:9, 11 vWA:15, 16 TPOX:8 Amelogenin:X D5S818:11, 13

Image / Description:   200倍視野下
  100倍視野下
 

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 61: 967-978, 1978; In Vitro 15: 723-729, 1979
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