Cell Line



BCRC Number: 60356
Name: NTERA-2 cl.D1
Type: pluripotent human testicular embryonal carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 22 years
Gender: Male
Tissue: testis; malignant pluripotent embryonal carcinoma; from metastatic site: lung

Morphology: fibroblast
Split Ratio: cultures should be maintained at high density; seed new flasks at a density of at least 5 x 106 viable cells per 75 sq.cm.flask
Mycoplasma Test: Negative
Cytogenetics: hypotriploid; modal chromosome number is 63
Virus resistance: UNTREATED CELLS: human cytomegalovirus (HCMV); human immunodeficiency virus (HIV-1, HTLV-III)
Virus susceptibility: RA and HMBA TREATED CELLS: human cytomegalovirus (HCMV); human immunodeficiency virus (HIV-1, HTLV-III)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: by scraping; trypsin-EDTA can be used but it is better to do so infrequently
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: this is a pluripotent human testicular embryonal carcinoma cell line derived by cloning the NTERA-2 cell line that was established from a nude mouse xenograft of the Tera-2 cell line; this clone differentiates along neuroectodermal lineages after to exposure retinoic acid (RA) or hexamethylene bisacetamide (HMBA).
Others: STR-PCR profile — D7S820:10, 12 CSF1PO:10, 12 TH01:9.3 D13S317:13 D16S539:11, 12, 13 vWA:18, 19 TPOX:8 Amelogenin:X, Y D5S818:9, 12

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Biochim. Biophys. Acta 948: 17-36, 1988; Differentiation 37: 73-79, 1988; Dev. Biol. 122: 21-34, 1987; Lab. Invest. 50: 147-162, 1984; Science 224: 159-161, 1984; Dev. Biol. 103: 285-293, 1984
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