Cell Line



BCRC Number: 60353
Name: LS1034
Type: human cecal carcinoma, Duke's Type C
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 54 years
Gender: unknown
Tissue: cecum; colorectal carcinoma

Morphology: epithelial
Split Ratio: 1:3
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: Negative; NOTE: The line has been cured of a previous mycoplasma infection.
Tumorigenic: yes, forms tumors in nude mice
Doubling time: 33 hrs

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: isolated from a primary tumor biopsy from a patient diagnosed with Dukes' Type C, moderately to poorly differentiated cecal carcinoma; more than 90% of the cells express surface CEA; the cells express the major histocompatibility (MHC) class I antigens and beta 2 microglobulin, but class II antigens, (HLA-DR, DQ, and DP) were not detected.
Others: STR-PCR profile — D7S820:8, 12 CSF1PO:11, 12 TH01:7 D13S317:12 D16S539:9, 11 vWA:17 TPOX:9, 11 Amelogenin:X, Y D5S818:12, 13

Image / Description:   100倍視野下
  200倍視野下
 

Biosafety Level: 1
Reference: Cancer Res. 52: 3705-3712, 1992 ; Lancet 340: 626-630, 1992: J. Biol. Chem. 270: 4971-4974, 1995
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