Cell Line



BCRC Number: 60352
Name: MeT-5A
Type: human mesothelium, transfected with SV40 early region genes
Species: Homo sapiens (human)
Gender: unknown
Tissue: mesothelium; epithelial; virus transformed

Morphology: epithelial
Split Ratio: 1:2 to 1:3
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: Negative
Tumorigenic: no; The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 95% M199 medium with 3.3 nM epidermal growth factor (EGF), 400 nM hydrocortisone, 870 nM insulin, 20 mM HEPES(see: Methods Cell Biol. 21B, 1980; Proc. Natl. Acad. Sci, 82: 3884-3888, 1985) + 5% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: isolated from pleural fluids obtained from non-cancerous individuals; the cells were transfected with the pRSV-T plasmid (an SV40 ori- construct containing the SV40 early region and the Rous sarcoma virus long terminal repeat) and cloned. construct containing the SV40 early region and the Rous sarcoma virus long terminal repeat) and cloned; the cells can be used to screen chemical and biological agents for activity as growth factor, carcinogens, mutagens, etc.
Others: STR-PCR profile — D7S820:10 CSF1PO:10, 12 TH01:6, 9.3 D13S317:7, 11, 13 D16S539:12 vWA:15, 18 TPOX:8 Amelogenin:X, Y D5S818:12

Image / Description:   200倍視野下
 

Biosafety Level: 1
Patent Statement: U.S. Pat. 4,885,238
Reference: U.S. Pat. 4,885,238 dated Dec. 5, 1989 ; Proc. Natl. Acad. Sci. USA 82: 3884-3888, 1985
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