Cell Line

BCRC Number: 60349 Add To Bookmark
Name: HCT 116
Type: human colorectal carcinoma
Species: Homo sapiens (human)
Age Stage: adult
Gender: unknown
Tissue: colon; colorectal carcinoma

Morphology: epithelial
Split Ratio: 1:3 to 1:8
Isoenzyme Analysis: NP, G6PD, MD,LD
Mycoplasma Test: Negative
Cytogenetics: near diploid; modal nunber=45
Tumorigenic: yes, nude mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% McCoy's 5a medium with 1.5 mM L-glutamine + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: the line is positive for transforming growth factor beta 1 (TGF beta 1) and beta 2 (TGF beta 2) expression; it has a mutation in codon 13 of the ras protooncogene, and can be used as a positive control for PCR assays of mutation in this codon; growth and plating efficiency are enhanced by using a feeder layer of murine fibroblasts
Others: STR-PCR profile — D7S820:11, 12 CSF1PO:7, 10 TH01:8, 9 D13S317:10, 12 D16S539:11, 13 vWA:17, 22 TPOX:8, 9 Amelogenin:X, Y D5S818:10, 11
Product Character: carcinoembryonic antigen (CEA) 1 ng per 106 cells per 10 days; keratin

Image / Description:   200倍視野下

Biosafety Level: 1
Reference: Cancer Res. 41: 1751-1756, 1981; J. Natl. Cancer Inst. 69: 767-771, 1982
Frozen Vial for all NTD$ 6000 Order this item

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