Cell Line

BCRC Number: 60348 Add To Bookmark
Name: DU 145
Type: human prostate carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 69 years
Gender: male
Tissue: prostate; carcinoma; from metastatic site: brain

Morphology: epithelial
Split Ratio: 1:4 to 1:6
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: Negative
Cytogenetics: hypotriploid
Tumorigenic: yes, in nude mice; forms adenocarcinoma (grade II) consistent with prostatic primary

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Isolated from a lesion in the brain of a patient with metastatic carcinoma of the prostate and a 3 year history of lymphocytic leukemia; the line is not detectably hormone sensitive, is only weakly positive for acid phosphatase and isolated cells from colonies in soft agar.Ultrastructural analyses of both the cell line and original tumor revealed microvilli, tonofilaments, desmosomes, any mitochondria, well developed Golgi and heterogenous lysosomes.
Others: STR-PCR profile — D7S820:7, 10, 11 CSF1PO:10, 11 TH01:7 D13S317:12, 13, 14 D16S539:11, 13 vWA:17, 18 TPOX:11 Amelogenin:X, Y D5S818:10, 13

Image / Description:   100倍視野下
  100倍視野下 高細胞密度
  200倍視野下 高細胞密度

Biosafety Level: 1
Reference: J.Natl. Cancer Inst. 66: 37-42, 1981; Int. J. Cancer 21: 274-281, 1978; Cancer Res. 37: 4049-4058, 1977
Frozen Vial for all NTD$ 6000 Order this item

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