Cell Line

BCRC Number: 60343
Name: SW620
Type: human colorectal adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 51 years; Dukes' type C
Gender: unknown
Tissue: colon; colorectal adenocarcinoma; metastatic site: lymph node

Morphology: epithelial
Split Ratio: 1:2 to 1:10
Mycoplasma Test: Negative
Cytogenetics: hyperdiploid; modal number = 50; range = 45 to 53
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

Incubation Temp(°C): 37°C
Incubation Condition: without CO2 in air
Culture Medium: 90% Leibovitz's L-15 medium + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: derived from a metastasis of the same tumor from which the SW480 was derived; the line consists mainly of individual small spherical and bipolar cells lacking microvilli; the cells synthesize only small quantities of carcinoembryonic antigen (CEA), and are highly tumorigenic in nude mice. The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative.The cells are positive for keratin by immunoperoxidase staining.The line was derived from a metastasis of the same tumor from which the SW480 (BCRC 60249) was derived.There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.N-myc oncogene expression was not detected.
Others: STR-PCR profile — D7S820:8, 9 CSF1PO:13, 14 TH01:8 D13S317:12 D16S539:9, 13 vWA:16 TPOX:11 Amelogenin:X D5S818:13
Product Character: carcinoembryonic antigen (CEA) 0.15 ng/106cells/10 days; transforming growth factor alpha; keratin; matrilysin

Image / Description:   200倍視野下

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 58: 209-214, 1977 ; J. Natl. Cancer Inst. 59: 221-226, 1977; J. Natl. Cancer Inst. 63: 635-645, 1979; Int. J. Cancer 41: 287-296, 1988
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