Cell Line

BCRC Number: 60341 Add To Bookmark
Name: P388D1 (IL-1)
Type: DBA/2 mouse macrophage, interleukin 1 (LAF) secreting
Species: Mus musculus (mouse)
Strain Ethnicity: DBA/2
Gender: unknown
Tissue: macrophage; monocyte; lymphoid tumor; methylcholanthrene induced

Split Ratio: 1:4 to 1:8
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: by scraping
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: this is a microphage-like cell line originally isolated from a methylcholanthrene-induced lymphoid neoplasm (P388) of a DBN/2 mouse; the cells produce IL-1 in response to LPS or phorbol myristic acetate (PMA); these cells phagocytose zymosan and latex beads;they are in antibody dependent cell mediated cytotoxicity systems; the cells are negative for surface immunoglobulin (sIg-).
Product Character: interleukin-1 induced by LPS or phorbol myristic acid (PMA, TPA); lysozyme

Image / Description:   200倍視野下

Biosafety Level: 1
Reference: J. Immunol. 122: 2167-2172, 1979; Ann. N.Y. Acad. Sci. 332: 539-549, 1979; J. Immunol. 119: 950-954, 1977; Cancer Res. 37: 546-550, 1977; J. Immunol. 114: 894-897, 1975; Manual of macrophage methodology.144; New York: Marcel Dekker, 1981:pp. 407-416
Frozen Vial for all NTD$ 6000 Order this item

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