Cell Line



BCRC Number: 60340
Name: P3X63Ag8
Type: mouse BALB/c myeloma, MOPC 21
Species: Mus musculus (mouse)
Strain Ethnicity: BALB/c
Gender: unknown
Tissue: myeloma; plasmacytoma; B lymphocyte

Morphology: lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: Negative
Cytogenetics: hyperdiploid; modal number = 65

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 2 x 105 cells/ml
Medium Renewal: add fresh medium (depending on cell density) every 2 to 3 days

Description: derived from the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma); the line secretes IgG1(gamma, kappa) of unknown binding specificity; the cells are resistant to 0.1 mM 8-azaguanine and die in HAT medium; the cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity.
Product Character: immunoglobulin; monoclonal antibody; isotype: IgG1; kappa light chain

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Exp. Cell Res. 60: 61-77, 1970; Nature 256: 495-497, 1975; Nature 249: 573-574, 1974; Nature 271: 249-251, 1978; Proc. Natl. Acad. Sci. USA 74: 2985-2988, 1977; Somatic Cell Genet. 3: 303-312, 1977; Eur. J. Immunol. 3: 135-140, 1973; FEBS Lett. 37: 311-316, 1973
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