Cell Line



BCRC Number: 60335
Name: PtK2
Type: marsupial kidney
Species: Potorous tridactylis/(potoroo) Synonym(s): rat kangaroo
Age Stage: adult
Gender: unknown
Tissue: kidney; normal

Morphology: epithelial
Split Ratio: 1:2 to 1:3
Mycoplasma Test: negative
Virus resistance: adenovirus 5; coxsackievirus B5; poliovirus 2
Virus susceptibility: coxsackievirus A9; herpes simplex; vaccinia; vesicular stomatitis (Ogden)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: derived from a kidney of an apparently normal adult male potoroo, Potorous tridactylis
Product Character: keratin

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Biosafety Level: 1
Reference: Genetics 51: 915,1965
Shipping/Field/Price:
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