Cell Line



BCRC Number: 60326 Add To Bookmark
Name: AML12
Type: mouse liver
Species: Mus musculus (mouse)
Strain Ethnicity: CD-1
Age Stage: 3 months
Gender: Male
Tissue: liver; normal; hepatocyte

Morphology: epithelial
Split Ratio: 1:4
Mycoplasma Test: negative
Tumorigenic: no; The cells were not tumorigenic in immunosuppressed mice, and do not form colonies or grow in soft agar.
Doubling time: 37 hrs

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 10 ug/ml insulin, 5.5 ug/ml transferrin, 5 ng/ml selenium, and 40 ng/ml dexamethasone + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: the AML12 (alpha-mouse-liver-12)cell line was derived from hepatocytes from the normal liver of a 3-month-old male homozygous mouse (CD1 strain, line MT42) transgenic for human transforming growth factor alpha (hTGF alpha); AML12 cells exhibit typical hepatocyte features by electron microscopy, such as peroxisomes and bile canalicular like structure;AML12 cells retain the capacity to express high levels of mRNA for serum (albumin, alpha 1 antitrypsin and transferrin) and gap junction (connexins 26 and 32) proteins, and contain solely isoenzyme 5 of lactate dehydrogenase;the cells express high levels of human TGF alpha and lower levels of mouse TGF alpha;expression of liver specific proteins decreases with time in culture, but is reactivated by growing the cells in serum free medium.
Product Character: albumin; human transforming growth factor alpha (TGF alpha); mouse TGF alpha

Image / Description:   100倍視野下高細胞密度
  100倍視野下
  200倍視野下
 

Biosafety Level: 1
Reference: Hepatology 22: 1279-1288, 1995; Proc. Natl. Acad. Sci. USA 91: 674-678, 1994
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