Cell Line



BCRC Number: 60323
Name: TF-1
Type: human bone marrow erythroleukemia
Species: Homo sapiens (human)
Strain Ethnicity: Japanese
Age Stage: 35 years
Gender: unknown
Tissue: bone marrow; erythroleukemia; erythroblast

Morphology: lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 1-5 ng/ml recombinant human GM-CSF + 10% fetal bovine serum
Growth Property: suspension
SubCulture Procedure: addition or replacement of fresh medium depending on cell density
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1-2 x 105 cells ml
Medium Renewal: every 2 to 3 days

Description: established from a heparinized bone marrow aspiration sample from a 35 year old Japanese male with severe pancytopenia; the cells are completely dependent on interleukin 3 (IL-3, also known as multi-colony stimulating factor or M-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth; the cells do not respond to interleukin 5 (IL-5); the cells respond to a variety of other lymphokines and cytokines such as interleukin 1 (IL-1), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 9 (IL-9), Interleukin 11 (IL-11), interleukin 13 (IL-13), stem cell factor (SCF), leukemia inhibitory factor (LIF) and nerve growth factor (NGF); the cells do not express glycophorin A or carbonyl anhydrase I; the morphological and cytochemical features, and the constitutive expression of globin genes, indicate the commitment of the cells to the erythroid lineage; hemin and delta-aminolevulinic acid induce hemoglobin synthesis, and TPA induces dramatic differentiation of the TF-1 cells into macrophage-like cells; the TF-1 cell line is unique because of its responsiveness to multiple cytokines and provides a good system for investigating the proliferation and differentiation of myeloid progenitor cells.
Others: STR-PCR profile — D7S820:12 CSF1PO:13 TH01:7, 9 D13S317:8, 9 D16S539:9, 12 vWA:15, 17 TPOX:8 Amelogenin:X, Y D5S818:13

Image / Description:   100倍視野下高細胞密度
 

Biosafety Level: 1
Reference: Int. Immunol. 3: 571-577, 1991; Blood 73: 375-380, 1989; J. Cell Physiol. 140: 323-334, 1989
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