Cell Line



BCRC Number: 60322 Add To Bookmark
Name: RAT2
Type: rat embryo, thymidine kinase mutant
Species: Rattus norvegicus (rat)
Strain Ethnicity: Fischer
Age Stage: embryo; fetus
Gender: unknown
Tissue: normal

Morphology: fibroblast
Split Ratio: 1:2 to 1:4
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 95% Dulbecco's modified Eagle's medium + 5% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: derived from the a 5-bromo-2'-deoxyuridine resistant strain of the Fischer rat fibroblast 3T3 like cell line, Rat1; Rat2 lacks detectable nuclear thymidine kinase, is highly transfectable by exogenous DNA and is phenotypically normal.

Image / Description:   200倍視野下高細胞密度
  200倍視野下低細胞密度
 

Biosafety Level: 1
Reference: Virology 113: 408-411, 1981
Shipping/Field/Price:
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