Cell Line

BCRC Number: 60321
Name: Mv 1 Lu
Type: mink lung
Species: Mustella vison (mink)
Age Stage: fetus
Gender: From Mixed Male and Female Organisms
Tissue: lung

Morphology: epithelial
Split Ratio: 1:3 to 1:4
Mycoplasma Test: negative
Cytogenetics: Both male and female diploid cells as well as pseudodiploid cells are present.
Virus resistance: adenovirus 5; coxsakievirus A9, B5; poliovirus 2
Virus susceptibility: herpes simplex; reovirus 3; vaccinia; vesicular stomatitis (Ogden)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: the Mv 1 Lu (NBL-7) cell line was initiated from trypsinized lungs of several nearly full-term, unsexed fetuses of the Aleutian mink; the cells are useful for focus forming assays for murine and feline sarcoma viruses

Image / Description:   200倍視野下高細胞密度

Biosafety Level: 1
Reference: Virology 60: 282-287, 1974
Frozen Vial for all NTD$ 6000 Order this item

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