Cell Line



BCRC Number: 60318
Name: SW 948
Type: human colon adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 81 years; Dukes' type C, grade III
Gender: Female
Tissue: colon; colorectal, adenocarcinoma

Morphology: epithelial
Split Ratio: 1:2 to 1:15
Mycoplasma Test: negative
Cytogenetics: hypotriploid
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

Incubation Temp(°C): 37°C
Incubation Condition: 0% CO2
Culture Medium: 90% Leibovitz's L-15 medium + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: The cells are positive for keratin by immunoperoxidase staining; the line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb and fos oncogenes; N-myc and sis oncogene expression were not detected.
Others: STR-PCR profile — D7S820:9, 11 CSF1PO:12 TH01:6, 9.3 D13S317:10, 11 D16S539:11, 12 vWA:16, 18 TPOX:8, 11 Amelogenin:X D5S818:11
Product Character: carcinoembryonic antigen (CEA) 7 ng/106 cells/10 days; colon specific antigen (CSAp) 750 units in 0.5 ml cell sonicate; keratin

Image / Description:   100倍視野下
  100倍視野下
  200倍視野下
 

Biosafety Level: 1
Reference: Cancer Res. 36: 4562-4569, 1976
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