Cell Line

BCRC Number: 60318
Name: SW 948
Type: human colon adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 81 years; Dukes' type C, grade III
Gender: unknown
Tissue: colon; coloretal, adenocarcinoma

Morphology: epithelial
Split Ratio: 1:2 to 1:5
Mycoplasma Test: negative
Cytogenetics: hypotriploid
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

Incubation Temp(°C): 37°C
Culture Medium: 90% Leibovitz's L-15 medium + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: The cells are positive for keratin by immunoperoxidase staining; the line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb and fos oncogenes; N-myc and sis oncogene expression were not detected.
Others: STR-PCR profile — D7S820:9, 11 CSF1PO:12 TH01:6, 9.3 D13S317:10, 11 D16S539:11, 12 vWA:16, 18 TPOX:8, 11 Amelogenin:X D5S818:11
Product Character: carcinoembryonic antigen (CEA) 7 ng/106 cells/10 days; colon specific antigen (CSAp) 750 units in 0.5 ml cell sonicate; keratin

Image / Description:   100倍視野下

Biosafety Level: 1
Reference: Cancer Res. 36: 4562-4569, 1976
Frozen Vial for all NTD$ 6000 Order this item

331, Shih-Pin Rd., Hsinchu 30062, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

web maintainance: +886-3-5223191 ext 593

Copyright © 2018.BCRC All rights reserved.The duplication or use of information and data such as texts or images or any linkage the website at the "bcrc.firdi.org.tw" is only permitted with the indication of the source or with prior approval by the BCRC(Bioresource Collection and Research Center).