Cell Line



BCRC Number: 60317 Add To Bookmark
Name: Hs 181.Tes
Type: human normal testis
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 14 weeks gestation
Gender: Male
Tissue: testis; normal

Morphology: fibroblast
Split Ratio: 1:2 to 1:3
Mycoplasma Test: negative
Cytogenetics: modal number = 46; range = 45 to 47

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:10, 11 CSF1PO:13 TH01:6, 9 D13S317:8, 13 D16S539:12 vWA:19 TPOX:8, 11 Amelogenin:X, Y D5S818:8, 12

Image / Description:   100倍視野下
 

Biosafety Level: 1
Reference: J. Tissue Culture Methods 11:223-227,1988
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