Cell Line



BCRC Number: 60316 Add To Bookmark
Name: 10P2
Type: mouse mast cell, transformed with Abelson murine leukemia virus
Species: Mus musculus (mouse)
Strain Ethnicity: (B10.BR X BALB/cAn)F1
Age Stage: 10 days gestation
Gender: unknown
Tissue: mast cell; Abelson murine leukemia virus transformed

Morphology: round (mast cell)
Split Ratio: maintain between 1 x 104 and 5 x 105 cells/ml; Do not allow the cell density to exceed 5 x 105 cells/ml.
Mycoplasma Test: negative
Receptor expressed: FcERI (Fc of IgE)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 0.05 mM 2-mercaptoethanol + 10% fetal bovine serum
Growth Property: suspension
SubCulture Procedure: addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1-2 x 104 cells/ml
Medium Renewal: every 2 to 3 days

Description: established by transformation of placental cells from a 10 day mouse embryo with A-MuLV; the cells are surface immunoglobulin negative, B23.1 positive and possess receptors for IgE; B220 antigen expression is inducible with BrdUrd and cytidine; this line represents a transformed stage of a very early hematopoietic precursor.

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Eur. J. Immunol. 15: 1136-1141, 1985; J. Immunol. 150: 4427-4437, 1993
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