Cell Line

BCRC Number: 60314 Add To Bookmark
Type: human teratocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Japanese
Age Stage: adult
Gender: unknown
Tissue: teratocarcinoma; pluripotent embryonal carcinoma

Morphology: epithelial
Split Ratio: 1:4 to 1:8
Mycoplasma Test: negative
Tumorigenic: yes, in nude mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA; cells will detach from surface of flask before confluency is reached.
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: add fresh medium at time of subculture

Description: established from a mediastinal mixed germ cell tumor; this pluripotent stem cell line is capable of somatic and extraembryonic differentiation; the undifferentiated cells are equivalent to an intermediate stage between seminoma and embryonal carcinoma; the the cells will differentiate in response to retinoic acid; the line was cured of a mycoplasma infection prior to deposit.
Others: STR-PCR profile — D7S820:10 CSF1PO:10, 12 TH01:7, 9 D13S317:11 D16S539:9, 12 vWA:14, 18 TPOX:8 Amelogenin:X D5S818:10, 13

Image / Description:   200倍視野下

Biosafety Level: 1
Reference: Lab Invest. 59: 328-336, 1988; Lab. InVest., 68: 220-232, 1993
Frozen Vial for all NTD$ 6000 Order this item

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