Cell Line



BCRC Number: 60311
Name: SW 48
Type: human colon adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 82 years; Dukes' type C, grade IV
Gender: Female
Tissue: colon; colorectal adenocarcinoma

Morphology: epithelial
Split Ratio: 1:2 to 1:6
Mycoplasma Test: negative
Cytogenetics: hyperdiploid
Tumorigenic: yes, in nude mice

Incubation Temp(°C): 37°C
Incubation Condition: without CO2 in air
Culture Medium: 90% Leibovitz's L-15 medium + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days; Do not fluid change for the first 3-4 days upon thawing.

Description: the cells produce little carcinoembryonic antigen (CEA) and are tumorigenic in nude mice; CSAp negative (CSAp-); the cells are positive for keratin by immunoperoxidase staining.
Others: STR-PCR profile — D7S820:9, 10 CSF1PO:9, 10 TH01:6, 9.3 D13S317:11, 12 D16S539:11, 13 vWA:18, 20 TPOX:8 Amelogenin:X D5S818:10, 14
Product Character: carcinoembryonic antigen (CEA) 0.6 ng/106 cells/10 days; keratin

Image / Description:   200倍視野下
  100倍視野下
  200倍視野下
  200倍視野下低細胞密度
 

Biosafety Level: 1
Reference: Cancer Res. 36: 4562-4569, 1976; J. Natl. Cancer Inst. 59: 221-226, 1977
Shipping/Field/Price:
Frozen Vial for all NTD$ 6000 Order this item

331, Shih-Pin Rd., Hsinchu 30062, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

web maintainance: +886-3-5223191 ext 593

Copyright © 2018.BCRC All rights reserved.The duplication or use of information and data such as texts or images or any linkage the website at the "bcrc.firdi.org.tw" is only permitted with the indication of the source or with prior approval by the BCRC(Bioresource Collection and Research Center).