Cell Line



BCRC Number: 60311
Name: SW 48
Type: human colon adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 82 years; Dukes' type C, grade IV
Gender: Female
Tissue: colon; colorectal adenocarcinoma

Morphology: epithelial
Split Ratio: 1:2 to 1:6
Mycoplasma Test: negative
Cytogenetics: hyperdiploid
Tumorigenic: yes, in nude mice

Incubation Temp(°C): 37°C
Incubation Condition: without CO2 in air
Culture Medium: 90% Leibovitz's L-15 medium + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days; Do not fluid change for the first 3-4 days upon thawing.

Description: the cells produce little carcinoembryonic antigen (CEA) and are tumorigenic in nude mice; CSAp negative (CSAp-); the cells are positive for keratin by immunoperoxidase staining.
Others: STR-PCR profile — D7S820:9, 10 CSF1PO:9, 10 TH01:6, 9.3 D13S317:11, 12 D16S539:11, 13 vWA:18, 20 TPOX:8 Amelogenin:X D5S818:10, 14
Product Character: carcinoembryonic antigen (CEA) 0.6 ng/106 cells/10 days; keratin

Image / Description:   200倍視野下
  100倍視野下
  200倍視野下
  200倍視野下低細胞密度
 

Biosafety Level: 1
Reference: Cancer Res. 36: 4562-4569, 1976; J. Natl. Cancer Inst. 59: 221-226, 1977
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