Cell Line



BCRC Number: 60310 Add To Bookmark
Name: RBL-2H3
Type: rat basophilic leukemia, chemically induced
Species: Rattus norvegicus (rat)
Strain Ethnicity: Wistar
Gender: unknown
Tissue: leukemia; basophil; chemically induced

Morphology: fibroblast
Split Ratio: 1:4 to 1:8
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 85% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 15% heat-inactivated fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: This is a basophilic leukemia cell line isolated and cloned from Wistar rat basophilic cells that were maintained as tumors; these cells have high affinity IgE receptors; they can be activated to secrete histamine and other mediators by aggregation of these receptors or with calcium ionophore.
Product Character: histamine (Fc-epsilon-RI)

Image / Description:   200倍視野下
  100倍視野下高細胞密度
  200倍視野下高細胞密度
 

Biosafety Level: 1
Reference: Nat. New Biol. 244: 73-76, 1973; Eur. J. Immunol. 11: 317-323, 1981; J. Tissue Culture Methods 11:223-227, 1988
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