Cell Line

BCRC Number: 60308 Add To Bookmark
Name: HOS
Type: human osteosarcoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 13 years
Gender: female
Tissue: bone; osteosarcoma

Morphology: mixed, fibroblast and epithelial like cells
Split Ratio: 1:3 to 1:6
Mycoplasma Test: negative
Cytogenetics: hyperdiploid

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: the cells exhibit flat morphology, low saturation density and low plating efficiency in soft agar and are sensitive to chemical and viral transformation.
Others: STR-PCR profile — D7S820:11, 12 CSF1PO:12 TH01:6 D13S317:12 D16S539:10, 13 vWA:18 TPOX:8, 11 Amelogenin:X D5S818:13

Image / Description:   200倍視野下

Biosafety Level: 1
Reference: Int. J. Cancer 15: 23-29, 1975; Int. J. Cancer, 16: 840-849, 1975; Cancer 27: 397-402, 1971.
Frozen Vial for all NTD$ 6000 Order this item

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