Cell Line



BCRC Number: 60307 Add To Bookmark
Name: SW 1116
Type: human colon adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 73 years; Dukes' type A, grade III
Gender: Male
Tissue: colon; colorectal adenocarcinoma

Morphology: epithelial
Split Ratio: 1:3 to 1:6
Mycoplasma Test: negative
Cytogenetics: hypotriploid, modal number=60
Tumorigenic: yes, in nude mice

Incubation Temp(°C): 37°C
Culture Medium: 90% Leibovitz's L-15 medium + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: negative for CSAp (CSAp-) and Colon antigen 3; positive for keratin by immunoperoxidase staining; positive for expression of c-myc, K-ras, H-ras, myb, sis and fos oncogenes; N-myc and N-ras oncogene expression were not detected; tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.
Others: STR-PCR profile — D7S820:12 CSF1PO:10, 11 TH01:6 D13S317:11, 14 D16S539:9, 12 vWA:14, 19 TPOX:8, 11 Amelogenin:X, Y D5S818:11, 12
Product Character: carcinoembryonic antigen (CEA) 2654 ng/106 cells/10 days; keratin

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Cancer Res. 36: 4562-4569, 1976
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