Cell Line

BCRC Number: 60304
Name: BT
Type: bovine turbinate
Species: Bos taurus (bovine)
Strain Ethnicity: Holstein
Age Stage: newborn, calf
Gender: unknown
Tissue: turbinate

Split Ratio: 1:3
Mycoplasma Test: negative
Virus susceptibility: bovine viral diarrhea; infectious bovine rhinotracheitis; some strains of parainfluenzavirus 3, bovine adenoviruses and bovine enteroviruses

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose and 1.0 mM sodium pyruvate + 10% horse serum; NOTE: Do not maintain the cells in serum of bovine origin.
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: the line should not be grown in medium containing serum of bovine origin; it is useful in growing bovine viral diarrhea virus (BVDV), infectious bovine rhinotracheitis virus (IBRV), some strains of parainfluenza 3 virus (PI-3V) and some types of bovine adenoviruses and enteroviruses.

Image / Description:   200倍視野下

Biosafety Level: 1
Reference: Virusforsch. 45: 285-289, 1974.
Frozen Vial for all NTD$ 6000 Order this item

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