Cell Line



BCRC Number: 60301
Name: IEC-6
Type: rat normal small intestine
Species: Rattus norvegicus (rat)
Gender: unknown
Tissue: small intestine; normal; epithelium

Morphology: epithelial
Split Ratio: 1:3 to 1:6
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 95% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and 1.0 mM sodium pyruvate supplemented with 0.1 Unit/ml bovine insulin + 5% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Cell growth is inhibited by cortisol; the cells express antigens characteristic of intestinal epithelia; the distribution stock is capable of about 15 additional population doublings.

Image / Description:   200倍視野下
  100倍視野下高細胞密度
  200倍視野下高細胞密度
 

Biosafety Level: 1
Reference: J. Cell Biol. 80: 248-265, 1979.
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