Cell Line



BCRC Number: 60299 Add To Bookmark
Name: HFL1
Type: human normal lung fibroblast
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: fetus
Gender: unknown
Tissue: lung; normal

Morphology: fibroblast
Split Ratio: 1:2 to 1:4
Mycoplasma Test: negative
Cytogenetics: diploid; modal number =46

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Ham's F12K medium + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: established from lung tissue of a 16-to 18-week-old human fetus; the line will undergo a total of at least 57 PDL
Others: STR-PCR profile — D7S820:9, 10 CSF1PO:10, 12 TH01:7, 9 D13S317:11, 12 D16S539:9, 11 vWA:17 TPOX:6, 9 Amelogenin:X, Y D5S818:12

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: J. Biol. Chem. 255: 5250-5260, 1980
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