Cell Line



BCRC Number: 60292
Name: MC 116
Type: human lymphoma
Species: Homo sapiens (human)
Gender: unknown
Tissue: lymphoma; undifferentiated; ascites; B lymphocyte

Morphology: lymphoblast
Split Ratio: maintain at or below 2-4 x 106 cells/ml
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: negative
Cytogenetics: aneuploid
Doubling time: 26 to 28 hrs

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2 in 100% humidity
Culture Medium: 80% RPMI 1640 medium + 20% fetal bovine serum
Growth Property: suspension
SubCulture Procedure: addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 3-5 x 105 cells/ml
Medium Renewal: every 2 to 3 days depending on cell density

Others: STR-PCR profile — D7S820:10, 13 CSF1PO:10, 12 TH01:9.3, 10 D13S317:12 D16S539:12 vWA:15, 17 TPOX:8, 10 Amelogenin:X, Y D5S818:12
Product Character: immunoglobulin (surface and secreted); isotype: IgM, lambda light chain

Image / Description:   100倍視野下
  200倍視野下
 

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 64: 465-476, 1980; J. Natl. Cancer Inst. 64: 477-483, 1980
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