Cell Line



BCRC Number: 60290 Add To Bookmark
Name: HeLa 229
Type: human cervical adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Black
Age Stage: 31 years
Gender: female
Tissue: cervix; adenocarcinoma

Morphology: epithelial
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: negative
Virus resistance: poliovirus 1.2.3
Virus susceptibility: vesicular stomatitis (Indiana); adenovirus 3

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids + 10% fetal bovine serum
Growth Property: adherent; can be adapted to growth in suspension
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: the line is a derivative of the parent HeLa line, but differs chiefly in its relative insusceptibility to poliovirus
Others: STR-PCR profile — D7S820:8, 12 CSF1PO:9, 10 TH01:7 D13S317:12 D16S539:9, 10 vWA:16, 18 TPOX:8, 12 Amelogenin:X D5S818:11, 12
Product Character: keratin

Image / Description:   200倍視野下
  100倍視野下高細胞密度
  200倍視野下高細胞密度
 

Biosafety Level: 2
Reference: Am. J. Pathol. 119: 361-366, 1985
Shipping/Field/Price:
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